Systems for cervical cytology reporting bethesda system tbs 1988. Specimens may be submitted as conventional smears, although the preferred method is the thinprep pap test. Ppt pap smears powerpoint presentation free to view. Download as ppt, pdf, txt or read online from scribd. Between 1991 and 2001, liquidbased cytology, automation, computerassisted imaging, and hpv testing were introduced and increasingly utilized in laboratories that offered cervical cytology testing. This monograph essentials of pap smear and breast cytology is prepared at the request of a large number of students in cytology who wish to have a small and concise book with numerous illustrations for easy reference during their laboratory training. Bethesda system for reporting cervical and vaginal cytology. Integrated cervical smear screening using liquid based. Annual pap smear after two negative lesbian, gay, bisexual, and other sexual minoritized youth. Fortis guide on pap smear a pap smear is a test your doctor does to check for signs of cancer of the cervix. From cytology to full molecular cervical screening ppt. Mojgan karimizarchi, fateme peighmbari, neda karimi, mitra rohi, and zohre chiti.
This technique was developed by george papanicolaou, the father of cytopathology. This is an atlas with more nearly 100 images, authentic taken from nci web atlas. A comparison of 3 ways of conventional pap smear, liquidbased cytology and colposcopy vs cervical biopsy for early diagnosis of premalignant lesions or cervical cancer in women with abnormal conventional pap test. Pap smears are not perfect for a high grade lesion, the sensitivity of a single pap smear is only 6080% estimated false negative rate is 3050%.
The bethesda system is now used to interpret pap smear findings. Cervical cytology and management of abnormal results revised 73120 page 2 of 7 infection sti prevention, contraception, and treatment of stis. Histopathology and cytopathology of the uterine cervix digital atlas. Primary care anal cancer with anal papanicolaou pap smears is controversial, we agree with the infectious disease society of america guidelines, which recommend anal pap tests for hivpositive msm, hivpositive women. Since then a lot of countries in the western world has switched from conventional pap smear more details cps method to lbc, although amid contrasting results from various studies comparing the benefits of lbc with cps. To evaluate a new liquidbased cytology technique, papspin thermo shandon, pittsburgh, pennsylvania, u. Cervical cancer articles case reports symptoms treatment, india. Liquidbased cytology is a new method of preparing cervical. Cervical cancer basic facts on screening and the pap test what is cervical cancer. The pap smear is usually done in conjunction with a pelvic exam. Oral cytology oral cytology exfoliative cytology, pap smear journal of the american dental association oral cytology should be a part of every oral examination in which the dentist detects even the least suspicious lesion recommendations published 30 years ago. Pap smear introduction principle and method in brief bethesda outlines liquid based cytology. Conventional pap in a conventional pap smear, samples are smeared directly onto a microscope slide after collection.
The stiff yet flexible brush head is made of polypropylene and has a protective tip to enhance the overall collection. This method is used to differentiate cells in the smear preparation of various gynecological specimens pap smears, materials containing exfoliative cells and material from fine needle aspiration. The subject has been presented in a way which will help students reproduce in exams. Ppt pap smears powerpoint presentation free to view id. Cancer is a disease in which cells in the body grow out of control.
Main purpose of the test is to detect precancerous lesions called squamous intraepithelial lesion sil, which are often caused by sexually transmitted human papillomaviruses. Hpv is the leading etiologic agent in development of dysplasia hpv dna is found in 99% of invasive cervical cancer icc and 7595% of highgrade. Cells are scrapped or brushed by a qualified physician, physicians assistant, nurse, or technician. View and download powerpoint presentations on liquid based cytology ppt. Papanicolaou test also called pap smear, pap test, cervical smear, or smear test is a screening test used to detect potentially precancerous and cancerous processes in the the endocervical canal transformation zone of the female reproductive system. A pap test is a test to screen for cervical cancer or other cervical diseases. A pap smear is a simple, quick, and relatively painless screening test in the vast majority of cases, a pap test does identify minor cellular abnormalities before they have had a chance to become malignant and at a point when the condition is most easily treatable. Atlas on bethesda system for reporting cervical cytology. A comparison of 3 ways of conventional pap smear, liquid. Ppt liquid based cytology powerpoint presentation free. A pap smear also known as the pap test is a medical procedure in which a sample of cells from a womans cervix the end of the uterus that extends into the vagina. Conventional pap smear and liquid based cytology for cervical.
Lab supplies, lab equipment, laboratory supplies, encompass, scientific, preferred, vendors, filter paper, balances, mixers, centrifuge, chemicals, solvents, high. Second generation of liquidbased cytology 1 lgm international inc. In an effort to decrease the occasional false negatives in the pap smear and further increase the screened population, thinprep pap test tp, a fluidbased cytology collection method, has been developed. Testing for cervical cancer screening was introduced in 1947 by dr. Liquidbased cytology versus conventional cytology for. The pap smear is used to sample the female genital trac t for presence of cellular change s which may indicate cervical or uterine carcinoma or their precursors. Liquid based cytology free download as powerpoint presentation.
This video explains the reasons why it may be done and describes how the test is performed. Ppt gynecologic cytopathology powerpoint presentation. Ppap smear reportingap smear reporting the pap smear reporting classification has evolved and been refined over time. A pap smear, also called a pap test, is a procedure to test for cervical cancer. Accuracy single pap smear diagnostic sensitivity 60% false negative results upto25% due to. Screening programs using conventional cytology conventional pap smear cps have successfully reduced cervical cancer, but newer tests like liquidbased cytology lbc and human papillomavirus testing might enhance screening. Whether the pap smear is an adequate sample incidental findings such as evidence of infection evidence of lesions. Colposcopy and biopsy was carried out in 45 cases with abnormal cytology either on pap spin or conventional pap smear.
During a pap smear, your doctor takes a sample of cells from your cervix to be tested. Liquidbased, thinlayer preparation of cervical cytology specimens was a subsequent modification in technique. Ideal for cutaneous or subcutaneous masses since it avoids surface contamination. Find powerpoint presentations and slides using the power of, find free presentations research about liquid based cytology ppt. Adolescents and young women who have received the hpv vaccine should continue cervical cancer screening according to the current recommendations. Triaging pap cytology negative, hpv positive cervical.
Scribd is the worlds largest social reading and publishing site. Chlamydia testing using floq and uriswabs is also discussed. Edited by frappart l, fontaniere b, lucas e, sankaranarayanan r. Siobhan bourke discusses and demonstrates correct technique for taking cervical samples. At the end of the test or after every question if you want you will have the possibilty to view the correct answers, and in. Pap smear screening has been documented to decrease the incidence and mortality rate of cervical cancer. Cells are scrapped or brushed by a qualified physician, physicians assistant, nurse, or. Edited by frappart l, fontaniere b, lucas e, sankaranarayanan.
Oss, the netherlands, used first for a pap smear and. Jan 12, 2020 this technique was developed by george papanicolaou, the father of cytopathology. As the drawing shows, the cervix is the lower, narrower part of the uterus. Pap test collection guidelines for cytologic examination acceptable specimens for the pap test include cervical, cervical and vaginal, endocervical, vaginal andor vulvar scrapings or brushings. The papanicolaou test abbreviated as pap test, also known as pap smear, cervical smear, cervical screening or smear test is a method of cervical screening used to detect potentially precancerous and cancerous processes in the cervix opening of the uterus or womb or colon in both women and men. Liquidbased cytology lbc was introduced in mid1990s as an alternative technique to process cervical samples. Gynecologic cytopathology conventional pap smear historical overview cervical cytology practice. It was developed during a workshop sponsored by the national cancer institute at bethesda, maryland in the united states. The pap test pap smear, papanicolaou test is a screening test used in gynecology. An hpv test may be performed on the sample to check for the virus and to ascertain whether highrisk or lowrisk strains are present.
Conventional pap smear and liquid based cytology for. Introduction and an overview of the evolution of the pap smear to cervical cancer screening as it is today. Cervical cytology became the standard screening test for cervical cancer and premalignant cervical lesions with the introduction of the papanicolaou pap smear in 1941. A pap smear can collect abnormal cells that are then identified in a lab. Methods and findings a populationbased sample of 5603 women from medchal mandal in andhra pradesh. Interpretation of results pap test report view in chinese.
Papanicolaou stain an overview sciencedirect topics. Pap smear pap smear sampling of cervix involves scraping of cervical surface. Cytology samples can be collected from solid lesions by several techniques including. Many advanced technologies involving sampling and smear preparation, or screening quality control have been developed to reduce the false negative cases. Most information and illustrations in this book are extracted from the authors. The fda has approved thinprep as an alternative for the cp smear and approved claims that it is more. The test may also detect infections and abnormalities in the endocervix and endometrium.
Find powerpoint presentations and slides using the power of, find free presentations research about cytopathology ppt. A free powerpoint ppt presentation displayed as a flash slide show on id. Generally, an ebook can be downloaded in five minutes or less. View and download powerpoint presentations on cytopathology ppt. Cervical cytologic smears in pap solution vs thinprep. Bethesda system is a system for reporting cervical and vaginal cytology or pap smear results. However, there is no satisfying clinical management algorithm for women testing pap negativehpv positive. Classification of cervical cancer cells in pap smear screening test 1236 where, 1, 2, 1, 2 are mean and standard deviation values accrued in the x and y directions individually. It is this area that the sample is taken from for the pap smear. The pap smear is a screening test and should not be used as the sole means of detecting cervical cancer. Background while many studies have compared the efficacy of pap cytology, visual inspection with acetic acid via and human papillomavirus hpv dna assays for the detection cervical intraepithelial neoplasia and cancer, few have evaluated the program effectiveness. Residents ppap smearap smear bioline international. When these abnormal cells are present in the cervix, it is called cervical cancer, or cancer of the cervix.
Jan 15, 2019 a pap test is a test to screen for cervical cancer or other cervical diseases. Conventional pap smear cps examination has been the mainstay for early detection of cervical cancer. Infection by highrisk hpv types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 may be associated with precancerous lesions or cancer of the cervix. It involves collecting cells from the vagina and cervix the lower, narrow end of the uterus thats at the top of the vagina.
Pap smears, cytology and cchc lab work and follow up. Entropy is a measure of nonuniformity in an image based on. Aug 19, 20 a comparison of 3 ways of conventional pap smear, liquidbased cytology and colposcopy vs cervical biopsy for early diagnosis of premalignant lesions or cervical cancer in women with abnormal conventional pap test. Importance of papsmears in the cervical cancer screening confidential. Cervical cytology sensitivity and specificity overall sensitivity. Cervical cancer basic facts on screening and the pap test. It was first introduced in 1988 and later revised in 1991 and 2001.
The main aim of the present study was to assess the diagnostic accuracy of lbc versus cps using split samples. Effectiveness of via, pap, and hpv dna testing in a. Evolution of the pap smear to cervical cancer screening today. Screening with conventional pap testing should occur every year. Papanicolaou smear cytology is widely accepted as a valuable diagnostic tool in the detection of cervical cancer and has also been described to be useful in the diagnosis of external ocular tumors. Widespread introduction of the pap begins conventional pap. The current reporting system is the bethesda system, which was introduced in 19886 and later updated again in 1999 table 1. The pap smear has brought about a dramatic improvement in the prevention of cervical cancer in women worldwide. The paptest pap smear, papanicolaou test is a screening test used in gynecology. Regular pap smears every 2 yrs is very effective in detecting abnormalities that may lead to. The pap test and bethesda 2014 american cancer society. Cytology pap smeargynecological specimen principle.
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